Bulk RNA-seq investigating differential gene regulation across gata3 knockdown and rescue

Loss of the transcription factor Gata3 associates with the highly variable human birth defects HDR syndrome and microsomia, and can lead to disruption of the neural crest-derived facial skeleton. RNA was isolated from cranial neural crest cells of zebrafish embryos at 28 hours post fertilization in control, gata3 knockdown, and gata3 overexpression embryos. Goal of the study was to illustrate multiple potential pathways for Gata3 function, and demonstrate a systematic, unbiased process to identify modifiers of genotype-phenotype correlations.