Expression data of Tfe3/Tfeb- or GFP-knocked-down RAW264 macrophages treated with or without iron

In an attempt to elucidate the role of MiT/TFE families in the iron-mediated phenotypic changes of macrophages, we performed DNA microarray analysis using ferric chloride-stimulated RAW264 cells transfected with Tfe3/Tfeb or GFP. A number of genes upregulated by ferric chloride treatment in siGFP-transfected RAW264 cells were decreased in siTfe3/Tfeb-transfected cells, suggesting MiT/TFE families are involved in phenotypic changes of macrophages induced by iron. RAW264 macrophages transfected with or without siTfe3/Tfeb were stimulated with or without ferric chloride for 24 h. Microarray analysis was performed using SurePrint G3 Mouse 8x60K ver. 2 .0.