RNA sequencing (RNA-seq) to quantify on-target and off-target editing RNA Editing using stereopure AIMers

To evaluate the specificity of AIMers, we first conducted deep RNA sequencing (RNA-seq) to quantify on-target ACTB editing and off-target editing in primary human hepatocytes, in primary monkey hepatocytes, and in vivo monkey livers.To assess off-target editing for the whole transcriptome, we selected Mutect2 to call edited sites due to its sensitivity in detecting low frequency variants and its ability to prefilter variants that were in both mock and treated samples.