Bulk transcriptomic comparison between in vitro propagated DNGR-1-lineage traced cells and hippocampus derived neural stem cells

We compared by bulk RNA sequencing the transcriptomic profile of in vitro cultured DNGR-1-traced neural stem cells or their differentiated astrocytic progeny with similar cell fates generated from bonafide neural stem cells derived from the hippocampus. Passage 10 NSCs adherent cultures were generated from tdTomato+, EYFP+ sorted from neurospheres grown from spinal cords of DNGR-1CreROSALSLtdTomato ot DNGR-1CreROSALFLEYFP mice, respectively. Hippocampus derived adherent NSCs cultures were generated from the hippocampus of GlastCreHuweflROSALSLEYFP mice. NSCs seeded in adherent conditions (+eGFP +bFGF+laminin) were recovered in StemPro accutase after 4 days or induced to differentiate into astrocytes (+BMP4) for 6 days. Cells from either condition were lysed with RLT buffer supplemented with 2-mercaptoethanol and cells lysates stored at -80C. Three replicates for each cell fate (3x10_6 NSCs or astrocytes) generated from passages 10, 11 and 12 were sequenced..