目的：制备靶向组蛋白H3多位点瓜氨酸化（R2、R8、R17）的兔多克隆抗体，并利用多平台技术综合评价其特异性。方法：对组蛋白H3（含R2、R8、R17残基）的N端1-19氨基酸序列进行生物信息学分析。瓜氨酸肽（R2cit、R8cit、R17cit）和相应的非瓜氨酸化对照肽的化学合成。肽偶联到锁孔帽贝血蓝蛋白（KLH）用于新西兰白兔的免疫。ELISA检测抗体滴度。通过点印迹、蛋白质印迹和细胞

DataCite Commons2025-08-14 更新40

Primary antibodies employed in this study and their properties.

抗体特异性

免疫检测

mAb, mouse monoclonal antibody; pAb, rabbit polyclonal antibody; WB, Western blotting; IF, immunofluorescence.

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Microarray binding patterns of nine chosen and sequenced scFv clones (supernatants from bacterial expression) against 25 glycated epitopes.

糖化蛋白识别

抗体特异性

Target nr# 1. Peptide 1, 2. Peptide 2, 3. Peptide 3, 4. Peptide 4, 5. Peptide 5, 6. Peptide 6, 7. Peptide 7, 8. Glucose-modified HSA, 9. Unmodified HSA, 10. Glyceraldehyde-modified BSA, 11. Glucose-mo

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Toxin specificity of the polyclonal antibody tested by direct ELISA.

抗体特异性

毒素免疫检测

Toxin specificity of the polyclonal antibody tested by direct ELISA.

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