Genomic and Transcriptional Characterisation of Carbapenem and Colistin Resistance Mechanisms in Klebsiella pneumoniae

Both carbapenem and colistin-resistance mechanisms are seen in Klebsiella pneumoniae. Carbapenem and colistin resistance mechanisms pose a significant threat to public and clinical health because carbapenem and colistin are last-line antibiotics for ESBL-positive Enterobacteriaceae. The aim of the study, therefore, is to study the resistance mechanisms of both carbapenem and colistin-resistant K. pneumoniae strains using genomics, and transcriptomics, and clustered-regularly interspaced short palindromic repeats (CRISPR). It will highlight the differentially expressed genes (DEGs) in the presence of the antibiotics. Clustered-regularly interspaced short palindromic repeats (CRISPR) associated proteins (Cas) will be used to knock out these genes to identify their function in antibiotic resistance.