Figure S1 - Outer Surface Protein C Is a Dissemination-Facilitating Factor of <em>Borrelia burgdorferi</em> during Mammalian Infection

Deletion of N-terminus 5-AA or 10-AA does not affect the surface location of OspC. The ΔospC/FL/1, ΔospC/ΔNt5/1 or ΔospC/ΔNt10/1 spirochetes were grown to late log phase (108 per ml) in BSK-H medium at 33°C. Spirochetes were incubated with OspC MAb, washed in PBS, probed with FITC-conjugated goat anti-mouse antibody, washed again before being placed onto slides. Differential interference contrast (left panel) and immunofluorescence images (right panel) were taken from the same field. Imagines were taken at ×400 magnification. (PDF)