CAIRA: Catalytic associated irregular residue analyser

For the semi-automated identification of potential cleavage
specificity-modulating SNVs we developed a program, called CAIRA
(Catalytic Associated Irregular Residue Analyser). Entering the UniProt ID
of a protease, CAIRA takes the predicted AlphaFold structure, generates a
user-definable radius (e.g. 20 Å) around the active site and filters the
COSMIC database for cancer-associated SNVs within this radius, which might
have cleavage specificity-modulating effects. CAIRA can be used with all
types of proteases (metallo, serine, aspartyl and cysteine proteases). To
further assess their potential impact, SNV-affected amino acids are
labelled in the protein structure of a downloadable pdb-file.