E0771 cultured mouse mammary tumor cell RNA-seq gene expression profiling: impact of activated cyclophosphamide versus interferon-beta, and the role of interferon signaling in cyclophosphamide responses

RNA-seq analysis of polyA-selected RNA isolated from cultured E0771 mouse breast cancer cells, with or without treatment with activated cyclophosphamide (4HC, 4-hydroperoxy-cyclophosphamide), interferon-beta, and antibody to the type-I interferon receptor IFNAR1. These RNA samples were part of a study where we investigated the role of type-I interferon signaling in therapeutic responses to cyclophosphamide in mouse models of breast cancer [Vergato et al, Canc Res Communic]. RNA-seq libraries were prepared from cultured tumor cell-extracted RNA obtained from n=3 independent cultures for each condition tested.