Precise diagnosis and typing of early-stage renal immunoglobulin-derived amyloidosis by label-free quantification of parallel reaction monitoring-based targeted proteomics

Untargeted proteomics based precise diagnosis and typing of amyloid deposits are crucial for guiding patient management. However, it may not be suitable for early-stage renal amyloidosis with minor damages. Here, we aimed to develop parallel reaction monitoring (PRM)-based targeted proteomics to achieve high sensitivity and specificity in identifying early-stage renal immunoglobulin-derived amyloidosis.This study demonstrates that the utility of these prioritized peptides in PRM-based targeted proteomics ensure highly sensitive and reliable for identifying early-stage renal amyloidosis. Owing to the development and clinical application of this method, rapid acceleration of the early diagnosis, and typing of renal amyloidosis is expected.