Expression data for human colon cancer cell line HCT116 transfected with siRNA targeting YAP1.

Yes-associated protein 1 (YAP1) is an effector of Hippo pathway, which is critical for regulating organ size, cell proliferation and tumor growth in mammals. YAP1 is known to be involved in tumorigenesis in several tissues, yet its role in colorectal cancer(CRC) is not established. To investigate the effect of YAP1 in CRC, we used microarrays to compared human colon cancer cell line HCT116 transfected with a control non-targeting siRNA to cells and transfected with siRNA targeting YAP1. Human colon cancer cell line HCT116 was transfected with a control non-targeting siRNA to cells or transfected with siRNA targeting YAP1 for 36 hours in DMEM medium (with 2% serum). Total RNA were extracted and detected by Affymetrix Human Genome U133 Plus 2.0 Array. 3 independent biological replicates were plated, transfected in parallel for each control and siRNA.