Gene expression in chow or HFD-fed OGG1-/- livers

Gene expression in livers of male wild-type (WT) and OGG1-deficient (Ogg1-/-) mice fed either a chow diet or a high-fat diet (HFD) were examined. Mice were fed the diet for 10 weeks prior to tissue collection and were 22 weeks of age at the time of tissue collection. 24 Total samples were analyzed. We generated the following pairwise comparisons using GeneSifter: WT Chow vs Ogg1-/- Chow; WT HFD vs. Ogg1-/- HFD using t-test followed by Benjamini and Hochberg correction. An adjusted p-value less than 0.05 was considered to be statistically significant.本研究检测了饲喂普通饲料（chow diet）或高脂饲料（high-fat diet, HFD）的雄性野生型（wild-type, WT）及OGG1基因缺陷型（OGG1-deficient, Ogg1-/-）小鼠的肝脏基因表达水平。所有小鼠在组织取材前均饲喂对应饲料10周，取材时小鼠周龄为22周，共纳入24份样本进行分析。我们借助GeneSifter软件开展了以下两组配对比较分析：野生型普通饲料组与OGG1基因缺陷型普通饲料组（WT Chow vs Ogg1-/- Chow）、野生型高脂饲料组与OGG1基因缺陷型高脂饲料组（WT HFD vs Ogg1-/- HFD）；分析方法为先采用t检验，随后进行本贾米尼-霍赫贝格（Benjamini and Hochberg）多重检验校正。校正后p值小于0.05即被认定为具有统计学显著性。