Transcriptomic analysis of resting or activated human CD226- and CD226+ effector memory CD8+ T cell subsets isolated from the peripheral blood of healthy donors.

We recently found that loss of the activating receptor CD226 (DNAM-1) was a critical mechanism affecting CD8+ T cell responsiveness to TCR stimulation. To better understand the molecular differences between CD226- and CD226+ CD8+ T cells, we decided to perform a global transcriptional analysis of purified CD226+ and CD226- CD8+ effector memory T cells before and after TCR activation using next generation RNA sequencing. We report here the total RNA sequencing of healthy donor resting CD226- and CD226+ CD8+ Tem cells (n=6/group) or activated (ACT, n=4/group) by α-CD2/CD3/CD28 for 24 hrs. Total RNA was extracted using RNeasy Micro kit (QIAGEN) and the sequencing was performed using next generation RNA sequencing