DNAseq of five generations of a selection experiment on two populations of Globodera pallida

We aimed to identify loci under selection for virulence in the potato cyst nematode Globodera pallida. Therefore, two G. pallida populations with suspected virulence, designated AMPOP02 and AMPOP10, were used in a selection experiment on the resistant potato variety Seresta. The selection experiment started in 2015, going through one generation per year, leading to fourth-generation selection populations at the end of 2018. The experiment was conducted in large pots, with a population that was as large as possible. After each generation cysts were saved for a later pot-experiment. This pot experiment was conducted in 2019 where the replication of these G. pallida populations on a set of potato cultivars (amongst others the non-resistant cultivar Desiree and the resistant cultivar Seresta). The first priority with the resulting generation was quantification and thereafter the material resulting from the Desiree and Seresta propagation was frozen in liquid nitrogen for DNA isolation. For each generation/potato cultivar combination three replicates were conducted. In total we had therefore 60 samples. Unfortunately, after DNA isolation, we had material of sufficient quantity and quality to conduct DNA sequencing on 45 samples.