Effect of Adgrd1 or Gpr39 knockdown during differentiation of adipose progenitor cell

Purpose: To explore the effect of Adgrd1 or Gpr39 knockdown in mouse primary adipose progenitor cell. Method: Primary adipose progenitor cell were isolated from inguinal white adipose tissue of mice. siRNA transfection was employed to achieve knockdown of Adgrd1 or Gpr39. 48 hours after differentiation induction, RNA were collected for sequencing. Results: cAMP and ERK pathway were significantly enriched by Adgrd1 and Gpr39 knockdown, respectively. Expression profiling by RNA-seq, profiling of mouse primary adipose progenitor cell after knockdown of Adgrd1 or Gpr39.