Blue laser light induces nuclear accumulation of Dorsal-GFP expressed together with CactDN-B-LID.
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The movie shows an embryo expressing Dorsal-GFP and CactDN-B-LID using the same conditions described for S1 Movie, from nc12 to nc14/gastrulation, importantly including 20 min high power blue light illumination (488nm, 8.6%) to initiate degron-mediated loss of CactDN-B-LID activity at nc13/14, resulting in Dorsal-GFP nuclear localization. Snapshots from the movie are shown in Fig 9E–9E“‘. (ZIP)
创建时间：
2021-05-17
