RNA m5C analysis in wildtype and NSUN2 knockdown T24 cell
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https://www.ncbi.nlm.nih.gov/sra/SRP212701
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资源简介：
RNA fragmentation and bisulfite conversion were performed. In brief, 200 ng of mRNAs along with 1 ng in vitro transcribed mouse dihydrofolate reductase (Dhfr) spike-in RNA were used. The bisulfite treated samples were desalted with Nanoseq with 3K omega 500/pk centrifugal devices (PALL). Sequencing was performed on an Illumina HiSeq X-Ten sequencing system. The m5C sites were called using meRanCall from meRanTK (FDR < 0.01). The differential m5C sites were detected Overall design: Examination of m5C changes in NSUN2 knockdown samples
创建时间：
2020-04-24
