Gene expression profile at single cell level of Wild type and Plagl1 knock out retina at P7

Plagl1 controls Muller glial cells proliferation in the retina. We used single cell RNA sequencing (scRNA-seq) to analyze the proliferative state of Muller glial cells and identify the signals that might be involved in this process Retinal cells were isolated from P7 wild type and Plagl1 KO retinas at P7. Dead cells removed by magnetic cell sorting and cells were analyzed using csRNAseqPlagl1可调控视网膜中米勒胶质细胞（Müller glial cells）的增殖。本研究采用单细胞RNA测序（scRNA-seq）分析米勒胶质细胞的增殖状态，并鉴定可能参与该调控过程的信号分子。研究人员于出生后第7天（P7）从野生型与Plagl1基因敲除（Plagl1 KO）的视网膜组织中分离视网膜细胞，通过磁性细胞分选去除死细胞后，采用csRNA-seq对细胞进行测序分析。