Cortical VIP neurons as a critical node for dopamine actions

Although dopamine modulates diverse cognitive processes of the prefrontal cortex including working memory, its underlying mechanisms are unclear. We investigated the roles of prefrontal vasoactive intestinal polypeptide (VIP)-expressing neurons, which are interneurons rich in D1 receptors (D1Rs), in working memory in mice engaged in a delayed match-to-sample task. VIP neurons conveyed robust working-memory signals and their inactivation profoundly impaired behavioral performance. Also, selective D1R knockdown in VIP neurons but not in pyramidal neurons impaired behavioral performance, indicating that VIP neurons are the primary mediators of dopamine effects on working memory. Additionally, we found that delay-period dopamine release dynamics vary depending on target location and that dopaminergic terminal stimulation increases prefrontal neuronal target selectivity in a laterality-dependent manner. These results indicate dopamine enhances working memory based on target laterality. These..., We implanted a GRIN lens with prism targeting layer 2/3 (VIP neuronal calcium imaging and dopamine-sensor imaging) or layer 5 (pyramidal neuronal calcium imaging) in the mPFC (2.0 mm ventral to brain surface). And recorded the Calcium signals and GRAB sensor signals in the mPFC during the delayed match-to-sample task. , , # Cortical VIP neurons as a critical node for dopamine actions

These datasets include raw calcium traces of VIP neurons and pyramidal neurons and raw GRAB-DA sensor traces of VIP-Cre mice in the mPFC during the delayed match-to-sample task.

## Description of the data and file structure

1. VIP recording data

1_VIP_Training, 208 cells

1_VIP_Well-trained, 684 cells

First column: Each cell calcium raw trace. Trials x time (refer time.mat file)

Second column: choice information (1, right correct; -1, left correct; 0.5, right error; -0.5, left error)

time, time 0 from delay onset (s), 4-s delay, delayed match-to-sample task.
2. GRAB-DA data

2_GRAB_Training, 7 mice

2_GRAB_Well-trained, 10 mice

2_GRAB_Well-trained (15 s), 7 mice

First column: Each mouse GRAB raw trace. Trials x time (refer time.mat file)

Second column: choice information (1, right correct; -1, left correct; 0.5, right error; -0.5, left error)

time, time 0 from delay onset (s), 4-s ...