Wheat leaf phosphoproteome - Integrative Network Analysis of the Signaling Cascades in Seedling Leaves of Bread Wheat by Large-Scale Phosphoproteomic Profiling

In this study, we performed a large-scale leaf phosphoproteomic analysis of two wheat genotypes HX10 and NC47 to explore the complex protein phosphorylation network of signaling and regulatory events affecting rapid vegetative growth in wheat. We used TiO2 affinity chromatography combined with LC-MS/MS and Maxquant software to identify phosphopeptides and phosphorylated sites. Finally, 2,336 phosphopeptides containing 2,734 phosphorylation sites were identified from the two wheat genotypes HX10 and NC47 in our study.