Hormone-regulated expansins - expression, localization and cell wall biomechanics in the control of Arabidopsis root growth

Expansins facilitate cell expansion by mediating pH-dependent cell wall (CW) loosening. However, the role of expansins in controlling CW biomechanical properties in specific tissues and organs remains elusive. To characterize its potential importance in controlling CW biomechanical properties, we overexpressed EXPA1 and generated Dex-inducible lines (8-4 and 5-4). We quantified the expression of genes from wild type and a Dex-inducible line (8-4). Total RNA was extracted from the roots of 7 days old seedlings. The experiment comprises four replicates for plants grown in control condition, treated for 3 hours with Dex or treated for 7 days with Dex. Overall design: Comparative gene expression analysis of RNA-seq data for wild type and Dex-inducible line under three conditions (control, 3h Dex treatment, 7 days Dex treatment).