PROTEIN PROFILING PECTORAL FIN-LOSS OF ADULT AFRICAN CATFISH Clarias gariepinus AND IT’S EXPRESSION OF T-Box5 PROTEIN

Various studies on aquatic commodity abnormalities have been studied since 48 years ago (Aulstad & Kittelsen, 1971) but the problem of skeletal abnormalities in fish cultivation is still a major obstacle for the aquaculture sector (Bardon et al, 2009; Estivals, et al, 2015) . Skeletal abnormalities are still a stagnant problem (Boglione et al, 2013).
African Catfish Clarias gariepinus is a popular species and has been widely cultivated in Indonesia since the 1980s. The hatchery technology has been mastered and is easy to do, making this fish more popular. In some centers of fish cultivation, abnormal fish are found in various forms of abnormalities. One form of abnormality that occurs is pectoral fin abnormality.
It was suspected that the loss of the C.gariepinus African pectoral fin organ from the origin of cultivation in Indonesia was caused by genetic factors. This refers to the knowledge that pectoral fin organogenesis involves genes that work together so that organs can grow in the right place and pair with symmetric bilateral forms. Searching for the activity of pectoral fin organ formation genes, it was found that the gene that determines the pectoral fin identity is the Tbx5 gene (Pi-Roiget al, 2014; Albalat et al, 2010; Hall, 2005; Minguillon et al, 2005; Begemann and Ingenham , 1999; Tamura et al, 1999).
This study aims to obtain the expression of protein T-Box5 (TBX5) of C.gariepinus African catfish that have pectoral fin morphological abnormalities. If TBX5 expression is different between abnormal fish and normal fish (control), then it can be proved that the pectoral fin morphological abnormality is a contribution from internal factors, in this case genetic factors. This research needs to be done so that the assessment of abnormalities in fish cultivation will progress, especially in the genetic field, so that a solution can be formulated to overcome the existing problems.