Optimal DivMelt analysis protocols for discrimination between recent and non-recent HIV infection.

Definitions and abbreviations: T1– temperature when melting began; T2– temperature when melting was complete; Theta 1– theta angle (°) for selection of T1; Theta 2– theta angle (°) for selection of T2; HI – hold interval (°C); SW – slope window (°C); SH - Shoulder ht threshold (%) tool (“off” indicates that the shoulder height threshold tool was not in use).aHIV genome regions (amplicons examined) have been described previously [5]. Briefly, the amplicons and the proteins coded by the corresponding HIV genomic regions are as follows: GAG1, p7; GAG2, p6 and transframe; POL, protease and reverse transcriptase; ENV1, HR1 region of gp41; ENV2, immunodominant region of gp41; and ENV3, HR2 region of gp41.