The original data on the enzymatic activity of GAGases and their mutants

This dataset comprises original experimental data on the enzymatic activity and structural-functional analysis of wild-type GAGases (GAGase II, III, VII) and their mutants. Specifically, it includes:

Wild-type GAGases: II, III, VII (expressed and purified)

Mutant Variants: Catalytic key site mutants (e.g., Asn, Tyr, His→Ala substitutions) and substrate-binding pocket mutants (e.g., Arg, Lys, His, Asn→Gly substitutions)

Experimental Data:

Activity Assays: Relative activity (absorbance in 232 nm) and HPLC raw data (raw chromatograms of reaction products (Analysis and separation by Superdex Peptide column).

Utility:
The dataset supports the study titled 'Crystal Structure and Catalytic Mechanism of PL35 Family Glycosaminoglycan Lyases with an Ultra-Broad Substrate Spectrum,' published in eLife.