ExtFig4B_RACE_STOML2_YKT6_R2_LG308.sgd
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HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
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Gaglia, Marta创建时间：
2023-01-19
