ZAP affects Zika virus RNA interactome - Table S1-ChIRP-MS data

One of the most recent advances in the analysis of viral RNA–cellular
protein interactions is the Comprehensive Identification of RNA-binding
Proteins by Mass Spectrometry (ChIRP-MS). Here, we used ChIRP-MS in
mock-infected and Zika-infected wild-type cells and cells knockout for the
zinc finger CCCH-type antiviral protein 1 (ZAP). We characterized
“ZAP-independent” and “ZAP-dependent” cellular protein interactomes
associated with flavivirus RNA and found that ZAP affects cellular
proteins associated with Zika virus RNA. The ZAP-dependent interactome
identified with ChIRP-MS provides potential ZAP co-factors for antiviral
activity against Zika virus and possibly other viruses. Identifying the
full spectrum of ZAP co-factors and mechanisms of how they act will be
critical to understanding the ZAP antiviral system and may contribute to
the development of antivirals.