Metagenomics data of the Antarctic Circumpolar Expedition (ACE)

218 samples for metagenomics analyses were collected at 34 stations during the ACE campaign in the Austral summer 2016-2017. 197 of the 218 samples were collected from Niskin bottles during CTD-rosette upcast and separated into three size fractions (0.2-3 µm, 3-200 µm, and 0.2-40 µm). The remaining 21 samples correspond to water pumped directly from the surface and separated into the same three size fractions (UDW samples). Samples were sent for DNA extraction and shotgun sequencing to Genoscope, the French National Platform for DNA Sequencing, following protocols used by Tara expeditions. Briefly, after filter cryogrinding, DNA was extracted using total RNA/DNA Purification and Nucleospin RNA/DNA Buffer Set (MACHEREY-NAGEL). Metagenomic libraries were prepared using the Illumina kit according to manufacturer instructions. DNA libraries were sequenced on a Novaseq 4000 instrument, with a target of 100M paired-end reads per library (2x150bp; 500bp insert size).