Changes in 3D genome organization of hepatocellular carcinoma (HCC) cells induced by lipid overload

We analyzed the 3D genome organization via the High-throughput/resolution chromosome conformation capture (Hi-C) data generated from lipid overload-induced HCC cells. Overall design: HepG2 cell lines were treated with 1% BSA (fatty acid-free) DMEM cell culture medium containing 120 µM palmitic acid (PA) and 240 µM oleic acid (OA) (for PA+OA group) or v/v 1/1000 DMSO (for mock group) for 48 hrs. The 3D genome organization via the High-throughput/resolution chromosome conformation capture (Hi-C) data generated from PA+OA or Mock-induced HCC cells were then analyzed.