Tabula Sapiens v2

Tabula Sapiens is a benchmark, first-draft human cell atlas of over 1.1M cells from 28 organs of 24 normal human subjects. This work is the product of the Tabula Sapiens Consortium. Taking the organs from the same individual controls for genetic background, age, environment, and epigenetic effects, and allows detailed analysis and comparison of cell types that are shared between tissues. Our work creates a detailed portrait of cell types as well as their distribution and variation in gene expression across tissues and within the endothelial, epithelial, stromal and immune compartments.We have built directly on our unique skills, experience, and data infrastructure from Tabula Muris and Tabula Muris Senis to create a high-quality human reference dataset and portal at a 10-fold larger scale from these prior efforts.A critical factor in the Tabula projects is our large collaborative network of PI’s with deep expertise at preparation of diverse organs, enabling all organs from a subject to be successfully processed within a single day. We have built the logistics and infrastructure capable of tracking hundreds of samples and thousands of 384-well plates from tissue through sample prep, library construction and on to sequencing and ultimately computational and expert cell annotation with tight quality control.Tabula Sapiens leverages our network of human tissue experts and a close collaboration with a Donor Network West, a not-for-profit organ procurement organization. We use their experience to balance and assign cell types from each tissue compartment and optimally mix high-quality plate-seq data and high-volume droplet-based data to provide a broad and deep benchmark atlas.With the second version, Tabula Sapiens 2.0, we have built an integrated map of 28 tissues collected across 24 donors. Nine new donors and four new tissues were collected and analyzed together with the original Tabula Sapiens 1.0 dataset. All tissues and organs, with the exception of the respective reproductive organs, were profiled from both male (n=11) and female (n=13) donors. The donor's age ranges from 22 to 74 years old, offering one of the most comprehensive molecular profiles of human tissues across the adult lifespan (7 donors under the age of 40, 11 donors between 40 and 60, and 6 donors over 60 years of age).Tabula Sapiens是首个基准级人类细胞图谱初稿，涵盖24名健康人类受试者的28个器官的超110万个细胞。该成果由Tabula Sapiens联盟（Tabula Sapiens Consortium）产出。通过取自同一受试者的器官样本，可控制遗传背景、年龄、环境及表观遗传效应的干扰，从而能够对不同组织中共有的细胞类型开展细致的分析与比较。本研究清晰刻画了各类细胞类型的特征，以及它们在不同组织间、在内皮、上皮、基质与免疫细胞组分中的分布模式与基因表达差异。本研究依托此前在Tabula Muris（小鼠细胞图谱）与Tabula Muris Senis（衰老小鼠细胞图谱）项目中积累的独特技术、经验与数据基础设施，构建了规模较此前成果提升10倍的高质量人类参考数据集与数据门户。Tabula系列项目的核心优势之一，是我们拥有由众多深耕多器官样本制备的首席研究员（Principal Investigator，PI）组成的大型协作网络，可确保单一名受试者的全部器官样本能在单日之内完成标准化处理。我们搭建了完整的物流与信息基础设施，可实现从组织样本制备、文库构建、测序，直至最终的计算分析与专家细胞注释全流程的追踪，覆盖数百份样本与数千块384孔板，并贯穿严格的质量控制体系。Tabula Sapiens项目依托我们的人类组织专家网络，并与非营利性器官获取组织西部供体网络（Donor Network West）开展深度合作。我们借助该组织的专业经验，对各组织组分的细胞类型进行校准与标注，并通过最优方式整合高质量平板测序（plate-seq）数据与高通量液滴测序数据，最终构建出覆盖全面且解析度精深的基准图谱。在第二版Tabula Sapiens 2.0中，我们构建了覆盖24名供体的28种组织的整合图谱。本次新增了9名供体与4种组织的样本，并与原始Tabula Sapiens 1.0数据集整合分析。除各自对应的生殖器官外，所有组织与器官均采集自男性（n=11）与女性（n=13）供体。供体年龄跨度为22至74岁，覆盖成年生命周期各阶段，是目前最为全面的人类组织分子图谱数据集之一（其中7名供体年龄低于40岁，11名介于40至60岁，6名高于60岁）。