Functional involvement of microRNAs in T cell ageing-related decline of CD28 expression. Functional involvement of microRNAs in T cell ageing-related decline of CD28 expression

MicroRNA expression profiling of T-cell clones with high or low population doublings as a longitudinal model of age-associated gene expression changes. Overall design: T cell clones were generated from phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC) by limiting dilution in the presence of IL-2 and pooled irradiated PBMC feeder cells. Three T cell clones from two healthy old and three T cell clones from one healthy young donor with low and high population doublings were selected for small RNA sequencing (n=12 samples).