Ecology of prophage-like elements in Bacillus subtilis at global and local geographical scale

Here, we combined computational and laboratory experiments to explore the abundance, distribution, and activity of prophage elements in Bacillus subtilis. Collection of B. subtilis soil isolates were treated with Mitomycin C, phage DNA was extracted and subjected to Illumina sequencing. Sequencing reads were mapped onto genome sequences of corresponding isolates to identify active prophage regions.