Data and code for: Relative testis size is associated with vagina length but not sperm storage traits in Galliformes

This dataset contains the data and code to replicate the analyses in “Relative testis size is associated with vagina length but not sperm storage traits in Galliformes.” The study examined relationships among sperm storage capacity, sperm storage tubule (SST) morphology, relative testis mass (a proxy for post-copulatory sexual selection), and sperm traits across 26 Galliforme species. The dataset includes four files: RepeatDF.csv (data for repeatability calculations); SummaryBothFolds.csv (raw species and fold-level data); SummarySpeciesAvgs.csv (species-level averages across both folds); and Analysis.R (code to replicate analyses). Data files contain measurements from female reproductive tracts, male testis mass, and sperm samples.
, For each species, one female was dissected; from each, two utero-vaginal junction (UVJ) folds (the SST-bearing region of the vagina) were analysed. Measurements from each individual include SST area and average tubule length measurements (from each UVJ fold), body mass, testis mass, vagina length, and sperm length (from 1–3 males per species; see dataset for details). A phylogenetic tree from Stein et al. (2015) is also required and available via Dryad (https://doi.org/10.5061/dryad.p2pn8).
, # Data and code for: Relative testis size is associated with vagina length but not sperm storage traits in Galliformes

Dataset DOI: [10.5061/dryad.z08kprrrf](10.5061/dryad.z08kprrrf)

## Description of the data and file structure

We dissected and measured the reproductive tract of 26 species of Galliformes, extracting and imaging the utero-vaginal junction (UVJ) and sperm storage tubules (using fluorescence microscopy). We also collected and measured testes and sperm of 20 species, which – combined with data from Liao et al (2019) – resulted in testes and sperm length measurements from 22 species of Galliformes (see methods in paper for further details).

The following three datasets and R script contain the data and code required to replicate analyses investigating the relationships between female reproductive tract morphology, sperm competition intensity, and sperm traits, whilst accounting for phylogenetic and allometric relationships. Phylogeny is provided by Stein et al., (2015)....,