Single-cell RNAsequencing of the ventral rhombomere 1 of the developing mouse [10X Genomics]

Sequencing of the mouse ventral rhombomere 1 tissues and cells to characterize the Tal1-dependent and -independent neuronal precursor groups. The data allowed to describe the cell types in the R1 brain region, and map gene expression dynamics during bifurcation of the GABAergic and glutamatergic lineages. Our results provide a framework for understanding the development of cellular diversity in the tegmental nuclei. Unlabelled single cells were dissociated from selected brain regions and collected on 10xgenomics Chromium platform.本数据集源自对小鼠腹侧菱脑节1（ventral rhombomere 1，R1）的组织与细胞的测序工作，旨在表征依赖Tal1与不依赖Tal1的神经元前体细胞群。该数据集可用于解析R1脑区的细胞类型，并绘制γ-氨基丁酸能（GABAergic）与谷氨酸能（glutamatergic）细胞谱系分化分支过程中的基因表达动态变化图谱。本研究结果为理解被盖核内细胞多样性的发育机制提供了理论框架。未标记的单细胞从选定脑区解离后，通过10x Genomics Chromium平台完成样本收集。