Whole-genome sequencing of new Trichoderma harzianum, Trichoderma atroviride, and Trichoderma reesei strains.

Members of the genus Trichoderma have been widely used as biofungicides, biofertilizers, and as model fungi for the industrial production of carbohydrate-active enzymes (CAZymes). In this context, the study of Trichoderma genomes could expand the understanding of their ecological traits; thus, improving their biotechnological applications. Herein, using the PacBio Single Molecule, Real-Time (SMRT) Sequencing (HiFi sequencing method), we performed whole-genome sequencing and assembly of Trichoderma species, including Trichoderma harzianum IOC-3844 (Th3844), Trichoderma harzianum CBMAI-0179 (Th0179), Trichoderma atroviride CBMAI-00020 (Ta0020), and Trichoderma reesei CBMAI-0711 (Tr0711). The resulting assembly revealed ab initio total gene structures prediction. The annotation of the predicted CDS sequences from the evaluated strains genes revealed CAZymes, distribution and orthology analyses.