Divalent cation effect on bacterial binding and preferential recognition of platelets over RBCs by Hsa-BR in whole blood.

(A) DL1 and DL1Δhsa binding to either platelets or RBCs were comparable in EDTA- or heparin-anticoagulated whole blood. Bar values = 100×[number of bacterium-bound platelets (or RBCs)/total number of platelets (or RBCs)]. (n = 3, SEM). (B) Quantification and comparison of Hsa-BR binding to platelets and RBCs in whole blood. Bar values = 100×[number of HsaBR-bound platelets (or RBCs)/total number of platelets (or RBCs)]. (n = 6, SEM). (C) Q1 shows mainly RBCs, and Q4 platelets. Only 2° antibody, anti-GST-APC, alone was added in whole blood. (D–F) 6 to 96 pmol of Hsa-BR was added to whole blood, followed by anti-GST-APC. ****P