AR inhibition prevents RWE-induced expression of cytokine and chemokines in mice lungs.
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Total RNA was isolated from homogenized lungs (n = 5–7), one microgram of total RNA from each sample was transcribed into first-strand cDNA and quantitative RT-PCR was conducted for selected genes using specific forward and reverse primers. The levels of RNA for the target sequences were determined by melting curve analysis. The values presented here are fold-change over the control. RWE, ragweed pollen extract; ARI, aldose reductase inhibitor.
创建时间：
2015-12-02
