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During late anaphase, Tem1 (green) localized to the spindle pole body (SPB) in cdc15-2 bfa1Δ cells grown at the restrictive temperature. DAPI (blue) and DIC (hidden) are also shown. Compare to image C
Werner Syndrome (WS) is a human genetic disorder with many features of premature aging. The gene defective in WS (WRN) has been cloned and encodes a protein homologous to several helicases, including
Conidia were inoculated into 200 ml medium at a concentration of 106 conidia/ml, and incubated at 37°C with shaking (200 rpm) for 36 h. The cell wall was extracted as described under Materials and Met