Rachel Horowitz-Scherer, Chris Woodcock (2010) CIL:773, Patiria miniata, sperm. CIL. Dataset

Phosphorus has a characteristic electron energy loss with an edge at 160 eV. The Zeiss 902 electron microscope permits images to be recorded at different energy losses which can be digitized and processed to yield a net phosphorus image. For nuclear chromatin, phosphorus is primarily located in DNA, allowing its distribution in chromatin fibers to be mapped. Electron energy loss (EELS) imaging is also known as electron spectroscopic imaging (ESI) and electron energy filtering TEM (EFTEM).
Starfish sperm chromatin, which has clearly defined chromatin fibers was fixed, embedded in Lowicryl at low temperature, thin sections prepared and imaged unstained. The 3 grouped images were recorded with a Zeiss 902 energy filtered TEM at 80KV using 5eV windows centered on energy loss values of 160, 120 (this image), and 110eV.

磷元素具有特征性的电子能级损失，其边缘位于160电子伏特。蔡司902型电子显微镜允许记录不同能量损失的图像，这些图像可以被数字化并处理，从而得到净磷图像。对于核染色质，磷主要位于DNA中，这使其在染色质纤维中的分布得以定位。电子能级损失成像（EELS）亦被称为电子能谱成像（ESI）以及电子能级筛选透射电子显微镜（EFTEM）。海星精子的染色质，其染色质纤维清晰可辨，经过固定、在低温下用Lowicryl包埋，制备了薄片并进行了无染色成像。三组图像使用蔡司902型能量过滤透射电子显微镜在80KV下，通过5eV的窗口，以160、120（此图像）和110电子伏特的能量损失值为中心进行记录。