Gene expression analysis of a nasopharynx adapted pneumococcal isolate and a lung adapted pneumococcal isolate in comparison to the wild-type ancestor D39 strain.

The gene expression profiles of a nasopharynx adapted pneumococcal isolate C20-3, and a lung adapted pneumococcal isolate P20-10, were compared to the D39N-wild-type ancestor strain. These evolved isolates were from an experimental evolution study with Streptococcus pneumoniae strain D39N in a mouse colonisation model, where we identified mutations arising in pneumococci experimentally adapted to nasopharynx and lung environments- doi.org/10.1093/molbev/msab018 Illumina sequencing of PCR amplicons from the nanB promoter, containing a poly-adenine signature, and from a promoter containing no homopolymer runs was also carried out to quantify mutation rate due to strand slippage