Solanum phureja Transcriptome or Gene expression. Solanum phureja

A synthetic autopolyploidy plant series O37 was developed from chromosome doublings of a monoploid (12 chromosome) plant derived from a Solanum phureja background. Monoploid (1x), diploid (2xR3 and 2xR5) and tetraploid (4x) plants were used for gene expression profiling. Total RNA was isolated from growing leaflets of six plants of each genotype at 20 day after planting. Total RNA was isolated and amplified from growing root tips of each genotype at 7 days after planting. Keywords: Genotype, Tissue type, Biorep, Loop design Overall design: Microarray hybridizations were performed in a loop design for three biological replicates.