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Summary of cloning efficiencies obtained with hCRY1AP-transgenic fibroblasts.

Figshare2015-12-02 更新2026-04-29 收录

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https://figshare.com/articles/dataset/_Summary_of_cloning_efficiencies_obtained_with_hCRY1_AP_transgenic_fibroblasts_/824666 数据链接链接失效反馈

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Summary of cloning efficiencies obtained with hCRY1AP-transgenic fibroblasts.

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2015-12-02

基因克隆方法比较

克隆效率评估

aThe competent cells [BL21(DE3)RIL] used had efficiency of 1×108 transformants/µg supercoiled pGEM3Z DNA. Cloning efficiency of four genes using column method and single tube method.

NIAID Data Ecosystem40

EGFP (A) and PCR identification (B) of goat fibroblast cell lines transfected by vector pBC1-hLF-Neo-IRES2-EGFP.

转基因细胞系构建

分子鉴定

(A) G418-resistant colony of fibroblast cells at fifth passage with EGFP fluorescence (×20). (B) M: DNA marker. Lanes 1 and 2: Non-transfected goat fibroblasts cells as negative controls, lanes 3 to 5

NIAID Data Ecosystem20

Developing a Triple Transgenic Cell Line for High-Efficiency Porcine Reproductive and Respiratory Syndrome Virus Infection

猪繁殖与呼吸综合征病毒模型

转基因细胞系构建

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most devastating pathogens in the swine industry worldwide. Due to the lack of robust cell lines and small animal models, the

Figshare2016-05-20 更新00

Comparison of cloning efficiencies of three methods producing different single-stranded overlaps.

分子克隆

克隆效率评估

Comparison of cloning efficiencies of three methods producing different single-stranded overlaps.

Figshare2016-10-27 更新30

Developing a Triple Transgenic Cell Line for High-Efficiency Porcine Reproductive and Respiratory Syndrome Virus Infection

猪繁殖与呼吸综合征病毒模型

转基因细胞系构建

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most devastating pathogens in the swine industry worldwide. Due to the lack of robust cell lines and small animal models, the

NIAID Data Ecosystem20

Summary of cloning efficiencies obtained with hCRY1AP-transgenic fibroblasts.

资源简介：

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