Humans PBMCs: Balanced constitution vs. Phlegm-dampness constitution. Humans PBMCs: Balanced constitution vs. Phlegm-dampness constitution

We investigated the expression profiles of lncRNAs and mRNAs in peripheral blood mononuclear cells (PBMCs) from subjects with 13 Phlegm-dampness constitution (PDC) and nine balanced Balanced constitution (BC). The differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs) were identified by the “limma” method. Goal was to determine whether long non-coding RNAs (lncRNAs) play a regulatory role in metabolic disease in subjects with PDC. Overall design: Two-condition experiment, BC vs. PDC. Peripheral blood mononuclear cells (PBMCs). Biological replicates:9 subjects with Balanced constitution (BC), 13 subjects with Phlegm-dampness constitution samples (PDC). This experiment was performed technically as a dual channel. Cy3 is used to the label of the PDC and the BC. Besides, RNA from all samples was as reference_RNA. And Cy5 is used to label the reference_RNA in each chip. Normalized log2 ratio (Cy3/Cy5) representing test/reference.