CRAC of yeast RNA polymerase II in anchor-away and auxin-degron strains.

We obtained RNA polymerase II occupancy profiles across the genome of S.cerevisiae strains: Abf1 anchor-away cells after addition of rapamycin for different time points (including no addition of rapamycin) and Rap1-AID auxin-degron cells after addition of auxin for different time points (including no addition of auxin) . This allowed us to compare polymerase occupancy profiles when these proteins are depleted from the nucleus or degraded.我们获取了酿酒酵母（S. cerevisiae）菌株的全基因组RNA聚合酶II（RNA polymerase II）占用谱：涵盖经雷帕霉素处理不同时长的Abf1锚定撤离细胞（包括未添加雷帕霉素的对照组），以及经生长素处理不同时长的Rap1-AID生长素降解标签细胞（包括未添加生长素的对照组）。借此我们得以对比这些蛋白从细胞核中耗竭或被降解时的聚合酶占用谱。