Protective mechanism of nicorandil on myocardial ischemia-reperfusion injury: a multi-omics study highlighting cyp4f5 as a key node

Myocardial I/R injury models were established using SPF-grade male Sprague-Dawley (SD) rats, randomly divided into control, I/R, and I/R+Nicorandil groups (n=6 per group). The I/R+Nicorandil group, nicorandil (5mg/kg) was injected intraperitoneally every day from 3 days before the establishment of myocardial I/R model. Myocardial injury was assessed through TTC staining, hematoxylin-eosin (HE) staining, and multi-omics analyses (transcriptome sequencing, proteomics, and metabolomics) to investigate alterations at genetic, protein, and metabolite levels.