RIP-Seq of Eif4a2 in mouse embryonic stem cell. RIP-Seq of Eif4a2 in mouse embryonic stem cell

RNA-immunoprecipitation can be used to identify transcripts bound by RNA binding proteins. Here we identified transcripts bound by the translation initiation factor Eif4a2 in ESCs. We found that several pluripotency transcription factor encoding mRNAs and factors regulating ESC differentiation show binding to Eif4a2 stronger than expected by transcript abundance. Overall design: Identification of transcripts bound by Eif4a2