Repli-seq sample preparation using cell sorting with cell-permeant dyes

DNA replication occurs in a defined temporal order known as the replication-timing (RT) program. RT is regulated during development in discrete chromosomal units, coordinated with transcriptional activity and 3D genome organization. Here, we developed a sample preparation method to measure RT genome-wide that does not require fixation. Overall design: Genome-wide replication timing profiles were constructed from the HepG2 cell line.