Expression of AID, αCT, Iμ-Cα transcripts in B-1 B cells from the oral-nasopharyngeal effector and inductive tissues of mice nasally immunized with TNP-LPS with/without nCT or nCT alone.a, b, c

aFive days after nasal immunization, B-1 B (CD5+ B220+) cells from the SMGs, NPs and NALT (as a positive control) were purified by FACS and were then subjected to semi-quantitative RT-PCR.bThe numbers are mean the expression percentages of the respective CSR-associated molecules when the density of β-actin expression on respective lymphoid tissues was calculated as 100 with ChemiDoc XRS Quantity one Analysis software (Bio-Rad).cThe values are presented as the mean ± SEM of 10 mice for each group and represent a total of five separate experiments.*p#p