The circadian clock of the bacterium B. subtilis evokes properties of complex, multicellular circadian systems

Luminometry datasets White, 96-well plates (Nunclon Delta, Thermo Fisher Scientific) were used, with each well inoculated with 5 × 105 cells. Plates were sealed with a transparent, evaporation-free cover (Optical Adhesive Covers, Applied Biosystems, Life Technologies). Cultures were exposed to light-darkness cycles as indicated in the manuscript, after which the cultures were released to constant conditions (either constant darkness or light). The temperature was kept constant at 27 °C. We measured bioluminescence (Berthold Centro LB960 XS3 or Berthold Mithras LB 940 Multimode Plate Reader) for 1 s each hour. All experiments were carried out in temperature-controlled incubators (MIR-154, Panasonic, Japan or Percival Intellus, Percival, USA). In entrainment experiments, the plates were ejected from the machine between readings for exposure to light. Datasets uploaded correspond to unprocessed, raw data. Luminometry data have been collected every hour and data analysis has been performed on detrended, normalised data as described in the manuscript.