Transcriptomic response dynamics of human primary and immortalized adrenocortical cells to ACTH and Angiotensin II

RNA sequencing provides a transcriptome-wide view of what processes are activated and repressed during steroidogenesis. Here we performed an RNA-seq time series on primary human adrenocortical cells and H295R cells stimulated with ACTH or AngII (forskolin for H295R). We found that the ligand-induced changes in gene expression largely involved the same genes and similar timing. While stimulus-induced expression changes in H295R cells are recapitulated by primary cells, there are also expression changes unique to primary cells. RNA sequencing of forskolin-treated H295R cells and AngII/ACTH-treated primary adrenocortical cells