Comparison of transcriptional responses to intracellular RNA and DNA between wild-type and RNase L KO cells. Mus musculus

Analysis of the response to intracellular nucleic acid ligands in wild-type versus RNase L KO cells showed that RNase L KO cells demonstrated an enhanced type I interferon to RNA, but not DNA ligands. Previous studies suggest that mRNAs may be a target of RNase L regulation. To investigate the extent that RNase L activation regulates the antiviral response by targeting of mRNAs, we performed microarray analysis comparing wild-type and RNase L KO cells treated with RIG-I ligand or CT DNA for 6 hours. Overall design: Total RNA was isolated from wild-type and RNase L KO BMDMs 6 hours following transfection of cells with either RIG-I ligand, CT DNA or left untreated. Each sample was treated and analyzed in triplicate.